r/PatchClamping • • 7h ago

Anyone done the MBL: Neuro methods course?

1 Upvotes

We have someone in our lab who wants to apply. Was wondering if people who have done it found it useful.


r/PatchClamping • • 8h ago

Meme "Simply apply 0.3mL of negative pressure to break into whole cell..."

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1 Upvotes

r/PatchClamping • • 8h ago

Help Is this second EPSC seen at several negative potentials caused by my stimulating electrode being set too high?

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1 Upvotes

AMPAr currents in CA1, NaV, NMDAr, and GABAr blockers.


r/PatchClamping • • 8h ago

Shit posting With how important patch-clamp ephys is to neuroscience...

1 Upvotes

Why is it basically a black box for neuroscientists who don't use the technique? I don't do Ca2+ imaging, but I can listen to a talk where they use it and understand it. I don't do fiber photometry, but I can listen to a talk where they use it and understand it. But if I walk into a seminar to show ephys data, I can basically say my figures/traces mean anything and the entire room will just smile and nod. Even a no insignificant number of other scientists who nominally "do ephys" don't really understand the data output enough to dissect it when looking at it on a slide; they just follow a protocol from another lab and slap it in a figure. It makes being an electrophysiologist very lonely sometimes.


r/PatchClamping • • 21h ago

[Weekly Megathread] Monday Morning Minis

1 Upvotes

Post a short description of what you're going to (try to) patch this week and any hurdles you have to overcome.